non-parametric anova with dunns multiple comparison test using graphpad instat (v. 3.10) Search Results


xlstat 2014.4.07  (Addinsoft inc)
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Addinsoft inc xlstat 2014.4.07
Xlstat 2014.4.07, supplied by Addinsoft inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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realtime statminer™ package  (Integromics)
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Integromics realtime statminer™ package
Realtime Statminer™ Package, supplied by Integromics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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realtime statminertm package  (Integromics)
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Integromics realtime statminertm package
Realtime Statminertm Package, supplied by Integromics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kyplot 5.0  (KyensLab Inc)
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KyensLab Inc kyplot 5.0
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galectin-3  (Galectin Therapeutics)
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Galectin Therapeutics galectin-3
Galectin 3, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r-nparcomp package  (RStudio)
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RStudio r-nparcomp package
R Nparcomp Package, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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drosophila activity monitors dams  (Trikinetics Inc)
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Trikinetics Inc drosophila activity monitors dams
(A) The <t>Drosophila</t> brain consists of neuronal and glial cell bodies arranged in a cortical rind (light green) enclosing dense synaptic neuropils (dark green). Within the Optic Lobe chiasm (OL-iCH), neuronal processes (dark green) are tightly associated with glial cells (yellow). Lysosomes (LysoTracker, red) are localized to cortical cell bodies (see Figure S1), while Ubiquitin (Ubi) aggregates are inside glia in gba1b mutant optic lobes (see Figure S2). Inside lysosomes, Gba1b degrades the lipid GlcCer into Cer (B-E″). LysoTracker labeling in brains from 2 day-old flies. (G-J″) Ubiquitin aggregates in the OL-iCh in 15–20 day-old flies. (B, G) Control. (B′, G′) gba1bΔ1/+ heterozygotes. (B″, G″) gba1bΔ transheterozygotes have enlarged lysosomes and accumulate Ubi aggregates in the OL-iCh (arrows). (C-C″, H-H″) RNA-interference, gba1bRNAi. Control (C, H), neural (C′, H′; nSyb-GAL4), and glial (C″, H″; repo-GAL4). (D-D″, I-I″) Somatic CRISPR, gba1bcKO. Control (D, I), neural (D′, I′; nSyb-GAL4), and glial (D″, I″; repo-GAL4). Both gba1bRNAi and gba1bcKO in glial cells but not neurons cause enlarged Lysosomes and Ubi aggregates. (E-E″, J-J″) Null gba1bΔ (E, J) can be rescued by nSyb::Gba1b (E′, J′; neurons) or by overexpressing UAS-Gba1bLAMP in glia (E″, J″; repo-GAL4). (F) Quantification of LysoTracker data. (K) Quantification of Ubi data. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons for normally distributed data, Kruskal-Wallis test for nonparametric data (E-E″, G-J). n>20 optic lobes. Data are represented as mean ± SEM. Scale bar: 25μm. See also Figure S1 and Figure S2, and Table S1 for genotypes.
Drosophila Activity Monitors Dams, supplied by Trikinetics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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version 2022.7.1.554  (RStudio)
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RStudio version 2022.7.1.554
(A) The <t>Drosophila</t> brain consists of neuronal and glial cell bodies arranged in a cortical rind (light green) enclosing dense synaptic neuropils (dark green). Within the Optic Lobe chiasm (OL-iCH), neuronal processes (dark green) are tightly associated with glial cells (yellow). Lysosomes (LysoTracker, red) are localized to cortical cell bodies (see Figure S1), while Ubiquitin (Ubi) aggregates are inside glia in gba1b mutant optic lobes (see Figure S2). Inside lysosomes, Gba1b degrades the lipid GlcCer into Cer (B-E″). LysoTracker labeling in brains from 2 day-old flies. (G-J″) Ubiquitin aggregates in the OL-iCh in 15–20 day-old flies. (B, G) Control. (B′, G′) gba1bΔ1/+ heterozygotes. (B″, G″) gba1bΔ transheterozygotes have enlarged lysosomes and accumulate Ubi aggregates in the OL-iCh (arrows). (C-C″, H-H″) RNA-interference, gba1bRNAi. Control (C, H), neural (C′, H′; nSyb-GAL4), and glial (C″, H″; repo-GAL4). (D-D″, I-I″) Somatic CRISPR, gba1bcKO. Control (D, I), neural (D′, I′; nSyb-GAL4), and glial (D″, I″; repo-GAL4). Both gba1bRNAi and gba1bcKO in glial cells but not neurons cause enlarged Lysosomes and Ubi aggregates. (E-E″, J-J″) Null gba1bΔ (E, J) can be rescued by nSyb::Gba1b (E′, J′; neurons) or by overexpressing UAS-Gba1bLAMP in glia (E″, J″; repo-GAL4). (F) Quantification of LysoTracker data. (K) Quantification of Ubi data. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons for normally distributed data, Kruskal-Wallis test for nonparametric data (E-E″, G-J). n>20 optic lobes. Data are represented as mean ± SEM. Scale bar: 25μm. See also Figure S1 and Figure S2, and Table S1 for genotypes.
Version 2022.7.1.554, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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group comparisons  (SYSTAT)
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SYSTAT group comparisons
(A) The <t>Drosophila</t> brain consists of neuronal and glial cell bodies arranged in a cortical rind (light green) enclosing dense synaptic neuropils (dark green). Within the Optic Lobe chiasm (OL-iCH), neuronal processes (dark green) are tightly associated with glial cells (yellow). Lysosomes (LysoTracker, red) are localized to cortical cell bodies (see Figure S1), while Ubiquitin (Ubi) aggregates are inside glia in gba1b mutant optic lobes (see Figure S2). Inside lysosomes, Gba1b degrades the lipid GlcCer into Cer (B-E″). LysoTracker labeling in brains from 2 day-old flies. (G-J″) Ubiquitin aggregates in the OL-iCh in 15–20 day-old flies. (B, G) Control. (B′, G′) gba1bΔ1/+ heterozygotes. (B″, G″) gba1bΔ transheterozygotes have enlarged lysosomes and accumulate Ubi aggregates in the OL-iCh (arrows). (C-C″, H-H″) RNA-interference, gba1bRNAi. Control (C, H), neural (C′, H′; nSyb-GAL4), and glial (C″, H″; repo-GAL4). (D-D″, I-I″) Somatic CRISPR, gba1bcKO. Control (D, I), neural (D′, I′; nSyb-GAL4), and glial (D″, I″; repo-GAL4). Both gba1bRNAi and gba1bcKO in glial cells but not neurons cause enlarged Lysosomes and Ubi aggregates. (E-E″, J-J″) Null gba1bΔ (E, J) can be rescued by nSyb::Gba1b (E′, J′; neurons) or by overexpressing UAS-Gba1bLAMP in glia (E″, J″; repo-GAL4). (F) Quantification of LysoTracker data. (K) Quantification of Ubi data. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons for normally distributed data, Kruskal-Wallis test for nonparametric data (E-E″, G-J). n>20 optic lobes. Data are represented as mean ± SEM. Scale bar: 25μm. See also Figure S1 and Figure S2, and Table S1 for genotypes.
Group Comparisons, supplied by SYSTAT, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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post hoc tukey  (GraphPad Software Inc)
99
GraphPad Software Inc post hoc tukey
(A) The <t>Drosophila</t> brain consists of neuronal and glial cell bodies arranged in a cortical rind (light green) enclosing dense synaptic neuropils (dark green). Within the Optic Lobe chiasm (OL-iCH), neuronal processes (dark green) are tightly associated with glial cells (yellow). Lysosomes (LysoTracker, red) are localized to cortical cell bodies (see Figure S1), while Ubiquitin (Ubi) aggregates are inside glia in gba1b mutant optic lobes (see Figure S2). Inside lysosomes, Gba1b degrades the lipid GlcCer into Cer (B-E″). LysoTracker labeling in brains from 2 day-old flies. (G-J″) Ubiquitin aggregates in the OL-iCh in 15–20 day-old flies. (B, G) Control. (B′, G′) gba1bΔ1/+ heterozygotes. (B″, G″) gba1bΔ transheterozygotes have enlarged lysosomes and accumulate Ubi aggregates in the OL-iCh (arrows). (C-C″, H-H″) RNA-interference, gba1bRNAi. Control (C, H), neural (C′, H′; nSyb-GAL4), and glial (C″, H″; repo-GAL4). (D-D″, I-I″) Somatic CRISPR, gba1bcKO. Control (D, I), neural (D′, I′; nSyb-GAL4), and glial (D″, I″; repo-GAL4). Both gba1bRNAi and gba1bcKO in glial cells but not neurons cause enlarged Lysosomes and Ubi aggregates. (E-E″, J-J″) Null gba1bΔ (E, J) can be rescued by nSyb::Gba1b (E′, J′; neurons) or by overexpressing UAS-Gba1bLAMP in glia (E″, J″; repo-GAL4). (F) Quantification of LysoTracker data. (K) Quantification of Ubi data. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons for normally distributed data, Kruskal-Wallis test for nonparametric data (E-E″, G-J). n>20 optic lobes. Data are represented as mean ± SEM. Scale bar: 25μm. See also Figure S1 and Figure S2, and Table S1 for genotypes.
Post Hoc Tukey, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethanol extracts of alpinia katsumadai seeds  (COSMAX Inc)
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COSMAX Inc ethanol extracts of alpinia katsumadai seeds
(A) The <t>Drosophila</t> brain consists of neuronal and glial cell bodies arranged in a cortical rind (light green) enclosing dense synaptic neuropils (dark green). Within the Optic Lobe chiasm (OL-iCH), neuronal processes (dark green) are tightly associated with glial cells (yellow). Lysosomes (LysoTracker, red) are localized to cortical cell bodies (see Figure S1), while Ubiquitin (Ubi) aggregates are inside glia in gba1b mutant optic lobes (see Figure S2). Inside lysosomes, Gba1b degrades the lipid GlcCer into Cer (B-E″). LysoTracker labeling in brains from 2 day-old flies. (G-J″) Ubiquitin aggregates in the OL-iCh in 15–20 day-old flies. (B, G) Control. (B′, G′) gba1bΔ1/+ heterozygotes. (B″, G″) gba1bΔ transheterozygotes have enlarged lysosomes and accumulate Ubi aggregates in the OL-iCh (arrows). (C-C″, H-H″) RNA-interference, gba1bRNAi. Control (C, H), neural (C′, H′; nSyb-GAL4), and glial (C″, H″; repo-GAL4). (D-D″, I-I″) Somatic CRISPR, gba1bcKO. Control (D, I), neural (D′, I′; nSyb-GAL4), and glial (D″, I″; repo-GAL4). Both gba1bRNAi and gba1bcKO in glial cells but not neurons cause enlarged Lysosomes and Ubi aggregates. (E-E″, J-J″) Null gba1bΔ (E, J) can be rescued by nSyb::Gba1b (E′, J′; neurons) or by overexpressing UAS-Gba1bLAMP in glia (E″, J″; repo-GAL4). (F) Quantification of LysoTracker data. (K) Quantification of Ubi data. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons for normally distributed data, Kruskal-Wallis test for nonparametric data (E-E″, G-J). n>20 optic lobes. Data are represented as mean ± SEM. Scale bar: 25μm. See also Figure S1 and Figure S2, and Table S1 for genotypes.
Ethanol Extracts Of Alpinia Katsumadai Seeds, supplied by COSMAX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) The Drosophila brain consists of neuronal and glial cell bodies arranged in a cortical rind (light green) enclosing dense synaptic neuropils (dark green). Within the Optic Lobe chiasm (OL-iCH), neuronal processes (dark green) are tightly associated with glial cells (yellow). Lysosomes (LysoTracker, red) are localized to cortical cell bodies (see Figure S1), while Ubiquitin (Ubi) aggregates are inside glia in gba1b mutant optic lobes (see Figure S2). Inside lysosomes, Gba1b degrades the lipid GlcCer into Cer (B-E″). LysoTracker labeling in brains from 2 day-old flies. (G-J″) Ubiquitin aggregates in the OL-iCh in 15–20 day-old flies. (B, G) Control. (B′, G′) gba1bΔ1/+ heterozygotes. (B″, G″) gba1bΔ transheterozygotes have enlarged lysosomes and accumulate Ubi aggregates in the OL-iCh (arrows). (C-C″, H-H″) RNA-interference, gba1bRNAi. Control (C, H), neural (C′, H′; nSyb-GAL4), and glial (C″, H″; repo-GAL4). (D-D″, I-I″) Somatic CRISPR, gba1bcKO. Control (D, I), neural (D′, I′; nSyb-GAL4), and glial (D″, I″; repo-GAL4). Both gba1bRNAi and gba1bcKO in glial cells but not neurons cause enlarged Lysosomes and Ubi aggregates. (E-E″, J-J″) Null gba1bΔ (E, J) can be rescued by nSyb::Gba1b (E′, J′; neurons) or by overexpressing UAS-Gba1bLAMP in glia (E″, J″; repo-GAL4). (F) Quantification of LysoTracker data. (K) Quantification of Ubi data. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons for normally distributed data, Kruskal-Wallis test for nonparametric data (E-E″, G-J). n>20 optic lobes. Data are represented as mean ± SEM. Scale bar: 25μm. See also Figure S1 and Figure S2, and Table S1 for genotypes.

Journal: Neuron

Article Title: Glial control of sphingolipid levels sculpts diurnal remodeling of circadian circuits

doi: 10.1016/j.neuron.2022.07.016

Figure Lengend Snippet: (A) The Drosophila brain consists of neuronal and glial cell bodies arranged in a cortical rind (light green) enclosing dense synaptic neuropils (dark green). Within the Optic Lobe chiasm (OL-iCH), neuronal processes (dark green) are tightly associated with glial cells (yellow). Lysosomes (LysoTracker, red) are localized to cortical cell bodies (see Figure S1), while Ubiquitin (Ubi) aggregates are inside glia in gba1b mutant optic lobes (see Figure S2). Inside lysosomes, Gba1b degrades the lipid GlcCer into Cer (B-E″). LysoTracker labeling in brains from 2 day-old flies. (G-J″) Ubiquitin aggregates in the OL-iCh in 15–20 day-old flies. (B, G) Control. (B′, G′) gba1bΔ1/+ heterozygotes. (B″, G″) gba1bΔ transheterozygotes have enlarged lysosomes and accumulate Ubi aggregates in the OL-iCh (arrows). (C-C″, H-H″) RNA-interference, gba1bRNAi. Control (C, H), neural (C′, H′; nSyb-GAL4), and glial (C″, H″; repo-GAL4). (D-D″, I-I″) Somatic CRISPR, gba1bcKO. Control (D, I), neural (D′, I′; nSyb-GAL4), and glial (D″, I″; repo-GAL4). Both gba1bRNAi and gba1bcKO in glial cells but not neurons cause enlarged Lysosomes and Ubi aggregates. (E-E″, J-J″) Null gba1bΔ (E, J) can be rescued by nSyb::Gba1b (E′, J′; neurons) or by overexpressing UAS-Gba1bLAMP in glia (E″, J″; repo-GAL4). (F) Quantification of LysoTracker data. (K) Quantification of Ubi data. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons for normally distributed data, Kruskal-Wallis test for nonparametric data (E-E″, G-J). n>20 optic lobes. Data are represented as mean ± SEM. Scale bar: 25μm. See also Figure S1 and Figure S2, and Table S1 for genotypes.

Article Snippet: Particle metrics were exported to excel and graphed and analyzed by ANOVA corrected via Tukey’s test for multiple comparisons in Prism GraphPad or Kruskal-Wallis test for comparisons of nonparametric data (generally Ol-iCh chiasm Ubi). . Sleep/Activity (DAM) assay and analysis We used Drosophila Activity Monitors (DAMs, Trikinetics).

Techniques: Ubiquitin Proteomics, Mutagenesis, Labeling, Control, CRISPR

Drosophila activity-monitor (DAM) assays for measuring sleep. Adult flies were analyzed from day 5 to day 10 on a 12-hour Light-Dark (LD) cycle. (A-A″) Fraction of flies asleep across ZT for male gba1bΔ flies (magenta) versus control males (green). (B-B″) Fraction of flies asleep across ZT for female gba1bΔ flies (magenta) versus control females (green). (C-C″) Fraction of flies asleep across ZT for glial gba1bcKO males (magenta) versus control males (green). (D-D″) Fraction of flies asleep across ZT for EG&PNG>gba1bcKO males (magenta) versus control males (green). (E-E″) Fraction of flies asleep across ZT for male gba1bΔ flies (magenta) versus rescue (EG&PNG>Gba1b) males (blue); control is overlaid (green, from A). (A, B, C, D, E) Fraction sleeping. (A′, B′, C′, D′, E′) Sleep per day. (A″, B″, C″, D″, E″) Total Sleep. Reducing Gba1b activity via either gba1bΔ, or repo-GAL4>gba1bcKO, or EG&PNG > gba1bcKO caused reduced sleep across all days and times. EG&PNG expression of Gba1b was sufficient to partially rescue gba1bΔ. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons or Welch’s ANOVA depending on equality of data variance. For nonparametric sleep bouts, Mann-Whitney U test was used. n>28 flies. Data are represented as mean ± SEM, as well as the IQR for the Total Sleep boxplots. See also Figure S6, and Table S1 for genotypes.

Journal: Neuron

Article Title: Glial control of sphingolipid levels sculpts diurnal remodeling of circadian circuits

doi: 10.1016/j.neuron.2022.07.016

Figure Lengend Snippet: Drosophila activity-monitor (DAM) assays for measuring sleep. Adult flies were analyzed from day 5 to day 10 on a 12-hour Light-Dark (LD) cycle. (A-A″) Fraction of flies asleep across ZT for male gba1bΔ flies (magenta) versus control males (green). (B-B″) Fraction of flies asleep across ZT for female gba1bΔ flies (magenta) versus control females (green). (C-C″) Fraction of flies asleep across ZT for glial gba1bcKO males (magenta) versus control males (green). (D-D″) Fraction of flies asleep across ZT for EG&PNG>gba1bcKO males (magenta) versus control males (green). (E-E″) Fraction of flies asleep across ZT for male gba1bΔ flies (magenta) versus rescue (EG&PNG>Gba1b) males (blue); control is overlaid (green, from A). (A, B, C, D, E) Fraction sleeping. (A′, B′, C′, D′, E′) Sleep per day. (A″, B″, C″, D″, E″) Total Sleep. Reducing Gba1b activity via either gba1bΔ, or repo-GAL4>gba1bcKO, or EG&PNG > gba1bcKO caused reduced sleep across all days and times. EG&PNG expression of Gba1b was sufficient to partially rescue gba1bΔ. *p<0.05, ****p<0.0001, ANOVA, Tukey’s multiple comparisons or Welch’s ANOVA depending on equality of data variance. For nonparametric sleep bouts, Mann-Whitney U test was used. n>28 flies. Data are represented as mean ± SEM, as well as the IQR for the Total Sleep boxplots. See also Figure S6, and Table S1 for genotypes.

Article Snippet: Particle metrics were exported to excel and graphed and analyzed by ANOVA corrected via Tukey’s test for multiple comparisons in Prism GraphPad or Kruskal-Wallis test for comparisons of nonparametric data (generally Ol-iCh chiasm Ubi). . Sleep/Activity (DAM) assay and analysis We used Drosophila Activity Monitors (DAMs, Trikinetics).

Techniques: Activity Assay, Control, Expressing, MANN-WHITNEY